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Thermo Scientific™ Pierce™ ECL 2 Western Blotting Substrate
Recommended HRP substrate for fluorescence imagers
Brand: Thermo Scientific™ 80196X3
Includes: Detection Reagent 1 and 2
Description
Pierce ECL 2 Substrate is a chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated antibodies and probes by Western blotting using X-ray film, CCD camera documentation systems or laser-based imagers. Pierce ECL 2 Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers.
Pierce ECL 2 substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. A broad dynamic range and excellent sensitivity combined with a long-lasting signal make Pierce ECL 2 Substrate a desirable Western blotting substrate.
Highlights:
- High sensitivity — detect targets down to the low-picogram level
- Long signal duration — sustained light output for as long as five hours
- More imaging options — X-ray, CCD or laser-based imagers
- More affordable — high quality and performance at a lower price than other suppliers' similar substrates
Specifications
| ECL Western Blot Substrate | |
| 30 Mini-gel blots, 3000cm2 of membrane | |
| 2°C to 8°C |
| 3 x 100 mL | |
| Detection reagent 1 and 2 |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Pierce ECL 2 Western Blotting Substrate
Signal Word
- Danger
Hazard Category
- Flammable liquid Category 2
- Serious eye damage/eye irritation Category 2
- Carcinogenicity Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H225-Highly flammable liquid and vapour.
- H319-Causes serious eye irritation.
- H351-Suspected of causing cancer.
- H335-May cause respiratory irritation.
Precautionary Statement
- P201-Obtain special instructions before use.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P235-Keep cool.
- P501-Dispose of contents/container to .
Supplemental information
- MIXTURE LIST-Contains : 1, 4-dioxane, ethanol
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Product Title
Thermo Scientific™ Pierce™ ECL 2 Western Blotting Substrate > For: 30 mini-gel blots; 3000cm2 of membrane
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