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R&D Systems™ Recombinant Human VEGF 165 Protein

Product Code. 18177350
10 μg, Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein., 10µg
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Quantity:
1 mg
10 μg
50 μg
500 μg
Formulation:
Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA with trehalose.
Unit Size:
10µg
1mg
500µg
50µg
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 1-6 ng/mL. The specific activity of recombinant human VEGF165 is approximately 1.7 x 103 U/μg, which is calibrated against recombinant human VEGF165 WHO Standard (NIBSC code: 02/286). Specific activity is for reference purposes only and is not routinely tested.
TRUSTED_SUSTAINABILITY

Specifications

Formulation Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Gene ID (Entrez) 7422
Molecular Weight (g/mol) observed_molecular_weight: 20-22 kDa, under reducing conditions. 39-42 kDa, under non-reducing conditions.
Name VEGF 165
Quantity 10 μg
Source Spodoptera frugiperda,Sf 21 (baculovirus)-derived human VEGF protein Ala27-Arg191
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
Structural Form Disulfide-linked homodimer
Conjugate Unconjugated
Recombinant Recombinant
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. Alternatively, reconstitute (at 100-500 μg/mL in sterile 4 mM HCl containing 0.1% human or bovine serum albumin.)
Purity or Quality Grade 97%, by SDS-PAGE with silver staining.
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