Reversibly stain proteins in polyacrylamide gels to identify bands to excise; then destain the gel pieces for analysis by mass spectrometry.
Brand: Thermo Scientific™ 24600
1633.41 NOK valid until 2025-03-21
Use promo code "24111" to get your promotional price.
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24111” to get your promotional price
Thermo Scientific™ Pierce Silver Stain for Mass Spectrometry is a complete kit for rapid and ultra-sensitive silver staining of proteins in polyacrylamide gels and efficient destaining of excised gel pieces for mass spectrometry analysis.
This silver staining kit for mass spectrometry (MS) bundles components of the high-performance Pierce Silver Stain Kit with optimized reagents to destain spots for subsequent in-gel tryptic digestion to recover peptide fragments for proteomics analysis. The resulting MS-compatible product and protocol deliver outstanding sensitivity and maintains favorable conditions for the high-yield recovery and identification (sequence coverage) of proteins by mass spectrometry. Silver staining of 2D gels is now an important intermediate step in a set of procedures that leads ultimately to identification of specific proteins in the proteome by mass fingerprinting methods. The Pierce Silver Stain protocol provides peak staining performance, flexibility, reliability and robustness for applications such as MALDI-MS.
Highlights:
Sensitive and fast staining – the low-background, easy-to-use silver stain provides subnanogram sensitivity, detecting proteins at less than 0.25ng per spot in 30 minutes after fixing
Robust – effective silver stain for even difficult-to-stain basic proteins, such as lysozyme (pI 10) and chymotrypsinogen A (pI 9.2), which are detectable at 0.2ng and 0.5ng, respectively
Simple, trouble-free spot preparation – stained spots in excised gel-pieces are easily destained and made ready for tryptic digestion in one hour
MS compatible – reagents and protocol are optimized to provide excellent performance in mass spectrometry following extraction of peptides from stained 1D or 2D gels (SDS-PAGE)
Complete kit – contains all reagents necessary for staining and destaining before in-gel proteolysis and peptide recovery for analysis by mass spectrometry
Convenient – kit-components are stable at room temperature; saves valuable refrigerator space and eliminates the need to equilibrate reagents before use
Includes:
Sensitizer, stain, developer and enhancer solutions for silver staining gels; Reagent solutions A and B for destaining excised gel pieces
| Kit Contents: • Silver Stain Sensitizer, 2 mL • Silver Stain Enhancer, 25 mL • Silver Stain Developer, 500 mL • Silver Stain, 500 mL • Silver Destain Reagent A, 4 mL • Silver Destain Reagent B, 14 mL Storage: Upon receipt store at ambient temperature |
|
| Colorimetric | |
| Silver Stain | |
| 1 L |
| In-Gel Detection | |
| Silver | |
| Pierce™ | |
| Protein |
Expires: 2025-03-21
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
For Research Use Only. Not for use in diagnostic procedures.
