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Thermo Scientific™ Pierce™ Premium Grade TCEP-HCl
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Optimize applications where product integrity and risk minimization are paramount with the specially characterized Thermo Scientific™ Pierce™ Premium Grade TCEP-HCl, our highest quality formulation of this disulfide reducing agent.
Thermo Scientific Pierce Premium Grade TCEP-HCl is our highest quality formulation of this disulfide reducing agent, specially characterized for applications where product integrity and risk minimization are paramount.
Compared to the standard grade product, Premium Grade SPDP provides more clearly defined quality and product support by including (a) increased analytical testing and product characterization, (b) greater batch-specific information and quality assurance review, (c) extensive lot sample retention and (d) change control notification. TCEP-HCl is pure, crystalline Tris(2-carboxyethyl)phosphine hydrochloride, a thiol-free compound that is highly effective at reducing protein and peptide disulfide bonds, as well as being odorless and stable. TCEP selectively and completely reduces even the most stable water-soluble alkyl disulfides over a wide pH range.
Highlights:
- TCEP – a thiol-free, odorless yet potent reducing agent for cleaving disulfide bonds
- High quality – identity and purity confirmed by several tests, including quantitative NMR
- Product integrity – enhanced level of testing and characterization compared to standard grade
- Lot retention – ample supply of past lots retained to ensure future process testing
- Change management – Change Control Notification (CCN) service
- Consistent manufacture – batch-specific manufacturing documentation review
Advantages of TCEP-HCl over other reducing agents:
- Odorless – Unlike DTT or BME, TCEP is odor-free, so reductions can be carried out conveniently on the bench top.
- Stable in air – The inherent stability of the TCEP moiety eliminates the need for any special precautions to avoid oxidation when handling, using or storing TCEP.
- Efficient – For most applications, 5 to 50mM TCEP provides sufficient molar excess to effectively reduce peptide or protein disulfide bonds within a few minutes at room temperature.
- Compatible – With TCEP, removal of the reducing agent is not necessary prior to most applications, (e.g. histidine-tagged protein purification, maleimide conjugations).
Stability in Solution
TCEP is stable in aqueous, acidic and basic solutions. When TCEP is dissolved directly in water, the resulting pH is approximately 2.5. Studies indicate that no change in concentration of TCEP occurs after 24-hour incubation at room temperature in 100mM HCl, 100mM NaOH, or in any of the following 50mM buffers: Tris·HCl (pH 7.5, 8.5 and 9.5), HEPES (pH 6.8 and 8.2), borate (pH 8.2 and 10.2) and CAPS (pH 9.7 and 11.1).5 Even after three weeks in these buffers, less than 20% of the TCEP was oxidized.

Specifica
Specifica
含量和储存 | Upon receipt store product at room temperature in sealed container to prevent oxidation. Product is shipped at ambient temperature. |
产品线 | Pierce™ |
形式 | Solid |
数量 | 1 g |
产品类型 | Disulfide Reducing Reagent |
Sicurezza e movimentazione

- Pierce Premium Grade, TCEP.HCl
- Pericolo
- Corrosione/irritazione della pelle Categoria 1B
- H314-Provoca gravi ustioni cutanee e gravi lesioni oculari.
- P260-Non respirare la polvere/i fumi/i gas/la nebbia/i vapori/gli aerosol.
- P280-Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.
- P284-¢Quando la ventilazione del locale è insufficiente! indossare un apparecchio di protezione respiratoria.
- P301+P310-IN CASO DI INGESTIONE: contattare immediatamente un CENTRO ANTIVELENI/un medico/
- P303+P361+P353-IN CASO DI CONTATTO CON LA PELLE (o con i capelli): togliere immediatamente tutti gli indumenti contaminati. Sciacquare la pelle/fare una doccia.
- P304+P340-IN CASO DI INALAZIONE: trasportare l'infortunato all'aria aperta e mantenerlo a riposo in posizione che favorisca la respirazione.
- P305+P351+P338-IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.
- P405-Conservare sotto chiave.
- P501b-Smaltire il prodotto/recipiente in conformità con le disposizioni locali/regionali/nazionali/internazionali.
- MIXTURE LIST-Contiene: Propanoic acid, 3,3',3''- phosphinidynetris-, hydrochloride
For Research Use Only. Not for use in diagnostic procedures.
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