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  5. 11809280

Thermo Scientific™ Metal Enhanced DAB Substrate Kit

Product Code. 11809280 Shop All Thermo Scientific Products
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Quantity:
275 mL of 1X working solution
Unit Size:
1 set
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

Special formulation of diaminobenzidine peroxidase substrate that uses cobalt and nickel chloride to yield intense color for IHC staining.

Thermo Scientific™ Pierce Metal-Enhanced DAB Substrate Kit uses cobalt and nickel chloride in a special formulation of diaminobenzidine peroxidase substrate that yields intense color for immunohistochemical staining.

The color produced by DAB (3,3'-diaminobenzidine) can be intensified by the addition of metals such as nickel, copper, silver and cobalt that form more dense complexes upon reaction in HRP-peroxide substrate reactions. The near-black signal (precipitate) is easy to photograph.

Highlights:

  • Incredible sensitivity – 50 times more sensitive than the traditional DAB method and 30 times more sensitive than other metal-intensified versions of DAB

  • Low background, high intensity – get a crisp dark brown-black precipitate and, even with the increased intensity, background is almost nonexistent

  • Six-hour stability – working solution is stable for more than six hours at room temperature

Includes:

Metal Enhanced DAB (10X), 25mL; Stable Peroxide Buffer, 250mL

TRUSTED_SUSTAINABILITY

Specifications

Detection Method Chromogenic, Colorimetric
Quantity 275 mL of 1X working solution
Content And Storage
  • Metal Enhanced DAB (10X), 25 mL (store at -20°C)
  • Stable Peroxide Buffer, 250 mL (store at 4°C)
Substrate DAB (Enhanced)
Substrate Type HRP (Horseradish Peroxidase) Substrate
Product Line Pierce™
For Use With (Application) Immunodetection, IHC (Immunohistochemistry)
Product Type DAB Substrate Kit

Safety and Handling

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Product Identifier
  • Metal Enhanced DAB Substrate Kit
 Signal Word
  • Danger
 Hazard Category
  • Flammable liquid Category 3
  • Acute toxicity Category 3
  • Skin corrosion/irritation Category 1 A
  • Serious eye damage/eye irritation Category 1
  • Respiratory sensitiser Category 1
  • Skin sensitiser Category 1
  • Carcinogenicity Category 1A
  • Reproductive toxicity Category 1B
  • Specific target organ toxicity after single exposure Category 1
  • Specific target organ toxicity after repeated exposure Category 2
  • LONG-TERM AQUATIC HAZARD Chronic 2
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • H301-Toxic if swallowed.
  • H311-Toxic in contact with skin.
  • H331-Toxic if inhaled.
  • H314-Causes severe skin burns and eye damage.
  • H318-Causes serious eye damage.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H317-May cause an allergic skin reaction.
  • H350-May cause cancer.
  • H360FD-May damage fertility. May damage the unborn child.
  • H370-Causes damage to organs.
  • H373-May cause damage to organs.
  • H411-Toxic to aquatic life with long lasting effects.
 Precautionary Statement
  • P201-Obtain special instructions before use.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P273-Avoid release to the environment.
  • P235-Keep cool.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P331-Do NOT induce vomiting.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305-IF IN EYES:.
  • P310-Immediately call a POISON CENTER/doctor/.
  • P501-Dispose of contents/container to .
 Supplemental information
  • MIXTURE LIST-Contains : methanol, ethanediol, N-carboxymethyliminobis(ethylenenitrilo)tetra(acetic acid), Cobalt chloride(Co

For Research Use Only. Not for use in diagnostic procedures.

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