Learn More
Thermo Scientific™ Pierce™ Classic IP Kit
Shop All Thermo Scientific ProductsDescription
Thermo Scientific™ Pierce Classic IP Kit uses high-quality Protein A/G agarose beads and microcentrifuge spin columns to accomplish traditional immunoprecipitation assays with ease.
Like traditional IP methods, the Pierce Classic IP protocol involves formation of antibody:antigen complexes in a sample solution and then capture of that complex to an IgG-binding protein that is covalently bound to beaded agarose resin (Protein A/G Agarose). After washing to remove nonbound (presumably undesired) components of the sample, the antigen and antibody are recovered from the beaded resin with elution buffer supplied in the kit. The entire procedure is performed in a microcentrifuge spin column, allowing solutions to be fully separated from the agarose resin upon brief centrifugation.
Highlights:
Assay consistency – spin columns eliminate resin loss, improve sample recovery and ensure efficient separation from resin
Easy protocol – immunoprecipitate (IP) target proteins from crude lysates in just four easy steps
Fast – complete an entire immunoprecipitation experiment in less than one hour
Economical – reuse the Protein A/G agarose resin for future immunoprecipitations
Complete – kit includes a compatible cell lysis reagent and sufficient components to perform at least 50 IP experiments
Includes:
Pierce Protein A/G Plus Agarose, lysis/wash buffer, conditioning buffer, Tris-buffered saline, elution buffer, lane marker sample buffer, control agarose resin, spin columns and collection tubes
Recommended for:
Routine, traditional immunoprecipitation experiments; Immunoprecipitation for analysis in nonreducing conditions
Specifications
Specifications
| Content And Storage | Sufficient For: At least 50 IP reactions with 10 μL resin • Protein A/G Plus Agarose, 0.55 mL • IP Lysis/Wash Buffer, 2 x 50 mL • Conditioning Buffer (100X), 5 mL • Tris-buffered Saline (20X), 25 mL • Elution Buffer, 50 mL • Lane Marker Sample Buffer (5X), 5 mL • Control Agarose Resin, 2 mL • Spin Columns and Collection Tubes Store at 4°C. |
| Assay | Affinity Purification |
| For Use With (Equipment) | Microcentrifuge |
| Product Line | Pierce™ |
| Quantity | 50 reactions kit |
| For Use With (Application) | Immunoprecipitation (IP) |
| Target | Not Target-Specific |
| Type | Immunoprecipitation/Co-Immunoprecipitation Kit |
| Sufficient For | 50 Reactions |
| Format | Kit |
Safety and Handling
- Pierce Classic IP Kit
- Warning
- Serious eye damage/eye irritation Category 2
- Flammable liquid Category 3
- H226-Flammable liquid and vapour.
- H319-Causes serious eye irritation.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P264-Wash hands thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P370+P378-In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish.
- P403+P235-Store in a well-ventilated place. Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : Sodium chloride, ethanol
For Research Use Only. Not for use in diagnostic procedures.
Your input is important to us. Please complete this form to provide feedback related to the content on this product.