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Thermo Scientific™ Pierce™ Classic IP Kit
Perform traditional immunoprecipitation assays with ease using high-quality Protein A/G agarose beads, microcentrifuge spin columns and buffers.
Brand: Thermo Scientific™ 26146
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Description
Thermo Scientific™ Pierce Classic IP Kit uses high-quality Protein A/G agarose beads and microcentrifuge spin columns to accomplish traditional immunoprecipitation assays with ease.
The Classic IP Kit provides all the necessary reagents, spin cups and collection tubes to easily perform successful immunoprecipitation (IP) experiments. The kit uses high-capacity Protein A/G agarose affinity resin for efficient binding of most species and subclasses of IP antibodies. The included IgG elution buffer provides milder and less denaturing recovery of antibody:antigen complexes than the traditional method of boiling in reducing sample buffer for SDS-PAGE, facilitating a greater variety of methods for subsequent analysis. The microcentrifuge spin column format helps to ensure effective washing and separation of samples from the beaded agarose affinity resin.Like traditional IP methods, the Pierce Classic IP protocol involves formation of antibody:antigen complexes in a sample solution and then capture of that complex to an IgG-binding protein that is covalently bound to beaded agarose resin (Protein A/G Agarose). After washing to remove nonbound (presumably undesired) components of the sample, the antigen and antibody are recovered from the beaded resin with elution buffer supplied in the kit. The entire procedure is performed in a microcentrifuge spin column, allowing solutions to be fully separated from the agarose resin upon brief centrifugation.
Highlights:
Assay consistency – spin columns eliminate resin loss, improve sample recovery and ensure efficient separation from resin
Easy protocol – immunoprecipitate (IP) target proteins from crude lysates in just four easy steps
Fast – complete an entire immunoprecipitation experiment in less than one hour
Economical – reuse the Protein A/G agarose resin for future immunoprecipitations
Complete – kit includes a compatible cell lysis reagent and sufficient components to perform at least 50 IP experiments
Includes:
Pierce Protein A/G Plus Agarose, lysis/wash buffer, conditioning buffer, Tris-buffered saline, elution buffer, lane marker sample buffer, control agarose resin, spin columns and collection tubes
Recommended for:
Routine, traditional immunoprecipitation experiments; Immunoprecipitation for analysis in nonreducing conditions
Specifications
| Sufficient For: At least 50 IP reactions with 10 μL resin • Protein A/G Plus Agarose, 0.55 mL • IP Lysis/Wash Buffer, 2 x 50 mL • Conditioning Buffer (100X), 5 mL • Tris-buffered Saline (20X), 25 mL • Elution Buffer, 50 mL • Lane Marker Sample Buffer (5X), 5 mL • Control Agarose Resin, 2 mL • Spin Columns and Collection Tubes Store at 4°C. |
|
| Microcentrifuge | |
| 50 reactions kit | |
| Not Target-Specific | |
| 50 Reactions |
| Affinity Purification | |
| Pierce™ | |
| Immunoprecipitation (IP) | |
| Immunoprecipitation/Co-Immunoprecipitation Kit | |
| Kit |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Pierce Classic IP Kit
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 2
- Flammable liquid Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
- H319-Causes serious eye irritation.
Precautionary Statement
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P264-Wash hands thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P370+P378-In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish.
- P403+P235-Store in a well-ventilated place. Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Sodium chloride, ethanol
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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