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OPTIZYME™ SalI, Fisher BioReagents™
Brand: Fisher BioReagents BP8013-1
This item is not returnable.
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Description
5'...G^T C G A C...3'
3'...C A G C T^G...5'
Supplied with: 10X OPTIZYME Buffer 3
Conditions for 100% Activity:
- 1X OPTIZYME Buffer 3: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 100mM NaCl and 0.1mg/ml BSA
- Incubate at 37°C
- Buffer 1: 0 - 20%
- Buffer 2: 0 - 20%
- Buffer 3: 100%
- Buffer 4: 0 - 20%
- Buffer 5: 20 - 50%
- 10mM Tris-HCl (pH 7.4 at 25°C),100 mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
- Low salt, high glycerol (>5%) concentrations, pH>8.0 or a large excess of enzyme may result in star activity.
- Supercoiled forms of pBR322 and pUC require 10-fold overdigestion with SalI to achieve complete digestion.
- Incubation at 25°C results in 50-75% activity.
Methylation Effects:
Dam: Never overlaps - no effect
Dcm: Never overlaps - no effect
CpG: Completely overlaps - blocked
- GTCGAC
Specifications
| 10 U/μL | |
| 37°C | |
| >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with TaqI | |
| 2000 U | |
| SalI |
| 10X OPTIZYME™ Buffer 3 | |
| 7.4 | |
| 10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 50% Glycerol | |
| G.TCGAC | |
| Liquid |
Safety and Handling
Product Identifier
- OPTIZYME
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