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OPTIZYME™ SalI, Fisher BioReagents™

Brand:  Fisher BioReagents BP8013-1

Product Code. 11954852

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Description

Description

5'...G^T C G A C...3'
3'...C A G C T^G...5'

Supplied with: 10X OPTIZYME Buffer 3
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 3: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 100mM NaCl and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 0 - 20%
  • Buffer 2: 0 - 20%
  • Buffer 3: 100%
  • Buffer 4: 0 - 20%
  • Buffer 5: 20 - 50%
Storage Buffer:
  • 10mM Tris-HCl (pH 7.4 at 25°C),100 mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 95% of the DNA fragments can be ligated and re-cut after a 50-fold over-digestion with SalI
  • Digestion of Agarose-embedded DNA:
  • A minimum of 5 units of SalI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
  • Notes:
    • Low salt, high glycerol (>5%) concentrations, pH>8.0 or a large excess of enzyme may result in star activity.
    • Supercoiled forms of pBR322 and pUC require 10-fold overdigestion with SalI to achieve complete digestion.
    • Incubation at 25°C results in 50-75% activity.
    Compatible Ends: Eco88I, SmoI, XhoI
    Methylation Effects:
    Dam: Never overlaps - no effect
    Dcm: Never overlaps - no effect
    CpG: Completely overlaps - blocked
    • GTCGAC
  • (Cleavage is blocked)
  • EcoKI: Never overlaps - no effect
  • EcoBI: Never overlaps - no effect

    • TRUSTED_SUSTAINABILITY
    Specifications

    Specifications

    10 U/μL
    37°C
    >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with TaqI
    2000 U
    SalI
    10X OPTIZYME™ Buffer 3
    7.4
    10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 50% Glycerol
    G.TCGAC
    Liquid
    Produktvorschläge

    Produktvorschläge

    Videos
    Sicherheit und Handhabung

    Sicherheit und Handhabung

    Produktidentifikator
    • OPTIZYME

    Sicherheitsdatenblatt (SDS)
    Dokumentation

    Dokumentation

    Zertifikate
    Aktionen

    Aktionen

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