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  6. Macherey-Nagel™ NucleoSpin™ Plasmid Transfection-grade, Mini kit for ultrapure plasmid DNA

Macherey-Nagel™ NucleoSpin™ Plasmid Transfection-grade, Mini kit for ultrapure plasmid DNA

Product Code. 15593626
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No. of Samples:
50
250
Unit Size:
250 reactions
50 reactions
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

A fast way to purify transfection-grade plasmid DNA using mini-format technology to reduce endotoxins from bacterial lysates. Complete kit includes mini spin columns for an ultra-fast workflow.

Endotoxins are co-purified during typical plasmid preparations from bacterial lysates. Since they interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. NucleoSpin™ Plasmid Transfection-grade kits are designed to reduce endotoxins from bacterial lysates.

  • Purifies high-copy plasmids for transfection
  • Novel technology to diminish endotoxin content (< 50 EU/μg DNA) for successful transfection
  • Rapid purification from E. coli via manual, vacuum or centrifugation processing
  • Ultra-fast procedure using mini spin columns simplifies the work-flow
  • Downstream applications: transfection of common cell lines (lipofection, electroporation)
  • Also suitable for standard molecular biology application, such as cloning and sequencing

Specifications

Bacterial Endotoxins <50 EU/ng DNA
Content And Storage Room temperature (18 - 25 °C)
Format Mini Spin Column
Isolation Technology Silica Membrane and Endotoxin-reduction Technology
Sample Type Bacterial Culture (E. coli, High-copy Plasmids)
Efficiency Binding capacity: 35 μg
Elution Volume Elution Volume: 30 - 50 μL
Final Product Type Transfection-grade plasmid DNA
For Use With (Equipment) Centrifuge, Vacuum manifold (e.g. NucleoVac 96)
For Use With (Application) Rapid purification of plasmids for transfection
Includes NucleoSpin Plasmid Transfection-grade Columns, Collection Tubes, Buffers, RNase A
No. of Samples 250
Purity A260/A280: 1.80 - 1.85
Sample Volume Up to 5 mL
Size < 15 kbp
Test Time 14 min/6 preps
Yield 15 - 30 μg
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Safety and Handling

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Signal Word
  • Danger
 Hazard Category
  • Flammable liquid Category 3
  • Acute toxicity Category 4
  • Skin corrosion/irritation Category 2
  • Serious eye damage/eye irritation Category 2
  • Respiratory sensitiser Category 1
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H336-May cause drowsiness or dizziness.
 Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
 Supplemental information
  • MIXTURE LIST-Contains : sodium hydroxide solution (diluted < 2 %), dodecyl sulfate,sodium salt/guanidine hydrochloride/trie
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