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Macherey-Nagel™ NucleoSpin™ Plasmid DNA
Prepare small scale high-purity plasmid DNA rapidly. NucleoSpin™ Plasmid Columns come with or without a cap lid.
Brand: Macherey-Nagel™ 740499.50
This item is not returnable.
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Description
Features:
- For rapid small scale preparation high-purity plasmid DNA
- Fast procedure – 6 Minipreps in 25 min.
- High capacity – up to 50μg of plasmid DNA
- Chaotropic salt wash buffer – extra pure DNA
Applications:
- High-copy plasmids from E. coli
- Low-copy plasmids, P1 constructs, or cosmids (increased buffer volumes required)
- Plasmid DNA from Gram-positive bacteria and plasmid DNA clean-up from reaction mixtures
- Supplementary protocol for M13 DNA available on request
- Typical downstream applications: cloning, sequencing, PCR, transformation, restriction analysis
Specifications
| Column | |
| DNA and RNA Sequencing | |
| No Lid | |
| Plasmid Columns | |
| 2 to 10 mL | |
| 25 min. |
| Silica Membrane Technology | |
| High-copy plasmids from E. coli | |
| 50 Preps | |
| 2 mL | |
| <50 kbp |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Danger
Hazard Category
- Flammable liquid Category 3
- Acute toxicity Category 4
- Skin corrosion/irritation Category 2
- Serious eye damage/eye irritation Category 2
- Respiratory sensitiser Category 1
- Specific target organ toxicity Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H336-May cause drowsiness or dizziness.
Precautionary Statement
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
Supplemental information
- MIXTURE LIST-Contains : sodium hydroxide solution (diluted < 2 %); guanidine hydrochloride; 2-propanol;Rnase
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