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Macherey-Nagel™ NucleoSpin™ 8 Trace, 8-well kit for DNA from forensic samples
Isolation of DNA from forensic samples in flexible 8‐well strip format.
4420.00 NOK - 18770.00 NOK
Specifications
| Content And Storage | 18°C to 25°C |
|---|---|
| Format | 8-well Strip |
| For Use With (Application) | Isolation of DNA |
| Product Code | Brand | No. of Reactions | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | No. of Reactions | Price | Quantity & Availability | |||||
|
12708552
|
Macherey-Nagel™
740722.1 |
96 Preps |
4420.00 NOK
96 reactions |
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|
12830276
|
Macherey-Nagel™
740722.5 |
480 Preps |
18770.00 NOK
Each |
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Description
Lysis is achieved by incubation of samples in a solution containing chaotropic ions in the presence of proteinase K. If subsequent separation of the lysate from the sample material is difficult, the lysate can be separated from the forensic material under vacuum or centrifugation by use of the NucleoSpin™ Trace filter plate (not included in the kits). Appropriate conditions for binding of DNA to the silica membrane in the NucleoSpin™ Trace binding strip/binding plate are created by addition of isopropanol to the lysate. The binding process is reversible and specific to nucleic acids. Contaminants are removed by two washing steps with ethanolic buffer. Pure genomic DNA is finally eluted under low ionic strength conditions in a slightly alkaline elution buffer.Typically yields of 1 to 2 μg genomic DNA can be purified from buccal swabs. The final concentration of eluted DNA is 10 to 20 ng/μL (depending on elution buffer volume). Typically, A260/280 is 1.8 to 1.9. The obtained DNA can be used directly as template for PCR.
Kit components
NucleoSpin™ Trace binding strips, MN wash plates, MN square well blocks, rack with tube strips, cap strips, self-adhering foil, buffers, proteinase K. NucleoSpin™ 8 Trace - NucleoSpin™ Trace binding plates, MN wash plates, MN square well blocks, racks with tube strips, cap strips, buffers, proteinase K.
- Suitable for manual and automated processing, processing possible under vacuum or by centrifugation
- 8 well strip format
- Typical yield: 1 to 2 μg DNA from buccal swabs
- Elution volume: 50 to 100 μL
- Silica membrane technology
- No use of organic solvents
- Highly pure nucleic acids suitable for all common downstream applications
- Preparation time: 30 min./8 strip
- Binding capacity: 20 μg
Specifications
| 18°C to 25°C | |
| 8-well Strip | |
| Isolation of DNA |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- NucleoSpin 8 Trace (12x8)
Signal Word
- Danger
Hazard Category
- Serious eye damage/eye irritation Category 2
- Respiratory sensitiser Category 1
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H335-May cause respiratory irritation.
Precautionary Statement
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
Supplemental information
- MIXTURE LIST-Contains : proteinase K
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