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Invitrogen™ Novex TNF- Mouse ELISA Kit
Designed to detect and quantify the level of mouse TNF-α in serum, plasma, and supernatant using 96-well plates and a microplate reader
4295.00 NOK - 6050.00 NOK
Specifications
| Sample Type | Plasma, Serum, Supernatant |
|---|---|
| For Use With (Application) | Colorimetric |
| Gene ID (Entrez) | 21926 |
| Kit Contents | Chromogen, Detector antibody, Diluents, Plate covers, Pre-coated plate, Standard, Stop solution, Streptavidin-HRP, Wash buffer |
| Precision | <3pg/mL |
Includes: Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.
Description
- The total assay incubation time is only 2.5 hour
- A monoclonal capture antibody specific for Mouse TNF-α has been coated onto the wells of the 96-well plate provided
- Samples, including a standard of known Mouse TNF-α content, controls, and unknowns, are pipetted into these wells followed by an addition of a biotinylated polyclonal detector antibody
- During the first incubation, Mouse TNF-α antigen binds to the immobilized capture antibody and to the solution phase biotinylated detector antibody on a second site
- After washing, a streptavidin-horseradish peroxidase (HRP) is added
- This binds to the biotinylated detection antibody to complete the four–member sandwich
- After a second incubation and washing to remove all unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color
- The intensity of this colored product is directly proportional to the concentration of Mouse TNF-α present in the original specimen and the optical density can be read on a standard microplate reader
The kit comes with a capture antibody precoated to the plate, eliminating an overnight coating process. The plates are machine coated to provide low variability well-to-well. Furthermore, the plate comes as removable 8-well strips allowing you to run as few samples as you want. Each ELISA kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency.
Apoptosis, Apoptosis Related Factors, Cell Analysis, Cell Signaling, Cell Viability, Proliferation and Function, Immunoassays, Ready-To-Use Immunoassay
Order Info
Shipping Condition: Wet ice
Specifications
| Plasma, Serum, Supernatant | |
| 21926 | |
| <3pg/mL | |
| Tumor necrosis factor |
| Colorimetric | |
| Chromogen, Detector antibody, Diluents, Plate covers, Pre-coated plate, Standard, Stop solution, Streptavidin-HRP, Wash buffer | |
| For research use only (RUO) | |
| 2.5 hr. |
For Research Use Only. Not for use in diagnostic procedures.
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