Invitrogen™ MultiShot™ FlexPlate TOP10 Competent Cells

Beschreibung

MultiShot FlexPlate TOP10 chemically competent E. coli cells are cloning competent cells that are pre-aliquoted in a 96-well PCR plate to increase transformation productivity. The FlexPlate format can be divided down to any combination of 8-well segments to allow for flexibility in transformation throughput.

Versatile TOP10 cloning capabilities
MultiShot FlexPlate TOP10 Competent Cells are the laboratory workhorse for chemical transformations as they are compatible with a broad spectrum of DNA types for transformation. The TOP10 strain is ideal for high-efficiency cloning and plasmid propagation, achieving transformation efficiency of >1 x 10⁸ cfu/μg with control plasmid DNA in FlexPlate format. It allows stable replication of high-copy number plasmids and is the same competent cell strain that come with many of our cloning kits.

TOP10 E. coli cells are genetically similar to the DH10B strain, but has been reported to be more resilient to stress conditions (osmotic shock and acidic pH stress). These cells carry mutations in the methylation-dependent restriction system (mcrA, mcrBC, and mrr), allowing the cloning of both prokaryotic and eukaryotic genomic DNA, as well as efficient plasmid rescue from eukaryotic genomes. In addition, recA1 and endA1 mutations increase insert stability and improve the quality of plasmid DNA prepared from minipreps.

MultiShot FlexPlate TOP10 Competent Cells offer:
• Transformation efficiencies of >1 x 10⁸ cfu/μg
• hsdR for efficient transformation of unmethylated DNA from PCR amplifications
• mcrA for efficient transformation of methylated DNA from genomic preparations
• lacZΔM15 for blue/white color screening of recombinant clones on plates containing either X-Gal or Bluo-Gal
• endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by endonuclease I
• recA1 for reduced occurrence of non-specific recombination in cloned DNA

Note: TOP10 cells do not require IPTG to induce expression from the lac promoter.

Increase transformation productivity with MultiShot FlexPlate format
The FlexPlate is a 96-well PCR plate that can be used in automated liquid handling systems or divided into 8-well segments for flexibility in transformation throughput. Addition of DNA to the MultiShot FlexPlate Competent Cells can be quickly performed with a multichannel pipet or a liquid handling system. After addition of DNA, the cells can be efficiently transformed by heat-shock using a water bath, heat block, or thermal cycler.

Key benefits of the MultiShot FlexPlate format
• Increased throughput—compatible with automated liquid handling platforms
• Flexible—transform in increments of 8 wells to entire 96-well plate
• Convenient—competent cells pre-aliquoted into 96-well plate, ready for transformation

Genotype
F^–mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK λ–rpsL(StrR) endA1 nupG

Find the strain and format that you need
• We offer many other E. coli strains and formats of chemically competent cells and electrocompetent cells to help meet specific transformation needs.
• For high-throughput transformation, choose from our collection of MultiShot formatted comp cells.
• Bacterial growth media is also available in various formats.