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Proteintech™ Mouse PCSK9 ELISA Kit
KE10050 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The PCSK9 ELISA kit is to be used to detect and quantify protein levels of endogenous PCSK9.
Brand: Proteintech™ KE10050-96T
Description
- The assay recognizes mouse PCSK9. An antibody specific for PCSK9 has been pre-coated onto the microwells.
- The PCSK9 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for PCSK9 is added to detect the captured PCSK9 protein.
- For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent.
- Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
- PCSK9, also named as NARC1 and PC9, belongs to the peptidase S8 family.
- PCSK9 is expressed and secreted by several tissues but principally by the liver, small intestines, and kidney(PMID 28587771). It may be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis.
- PCSK9 is a regulator of the LDL receptor and plasma cholesterol concentrations(PMID: 26293463).
Specifications
BC038085 | |
11.9 pg/mL | |
Sandwich ELISA, Double Antibody | |
Plasma, Serum | |
Mouse | |
Mouse | |
<10% | |
Blue ice | |
4.5 hr. |
62.5 to 4000 pg/mL | |
ISO 13485 | |
Coated ELISA Kit | |
DAB | |
100102 | |
Solid | |
<10% | |
-20°C to +4°C | |
Q80W65 |
Safety and Handling
- 96TEST Human IL-4 ELISA Kit
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 1
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H318-Causes serious eye damage.
- H319-Causes serious eye irritation.
- H332-Harmful if inhaled.
- H335-May cause respiratory irritation.
Precautionary Statement
- P201-Obtain special instructions before use.
- P202-Do not handle until all safety precautions have been read and understood.
- P234-Keep only in original container.
- P264-Wash skin thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P330-Rinse mouth.
- P362-Take off contaminated clothing.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine
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