Invitrogen™ LIVE/DEAD™ Fixable Yellow Dead Cell Stain Kit, for 405 nm excitation

Description

• Used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation
• Kit has been optimized and validated for use with a violet laser flow cytometer
• Conveniently packaged in 40-test vials to help ensure the stability and performance of the dye over time
• Amine reactive dyes in solution will lose their effectiveness over a short period of time, therefore it is recommended to completely use the vial once rehydrated
• If this is not possible, aliquot the vials in small volumes and store at -80°C, avoiding freeze-thaw cycles
• LIVE/DEAD™ Fixable Dead Cell Stains are available in a wide variety of colors to meet multi-color panel needs

Low compensation

• The LIVE/DEAD™ Fixable Yellow Stain was selected based on its fluorescent properties to minimize compensation between other violet dyes and dyes that excite off of the 488 nm blue laser
• The yellow-fluorescent reactive dye has an excitation maximum of ∼405 nm, but it is excited well with the 405 nm violet laser
• It has an emission maxima of ∼570 nm, so it can be collected in the second or third channel on most violet laser flow cytometers