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Invitrogen™ Human IL-6 ELISA Kit
ELISA
Brand: Invitrogen™ EH2IL6
4160.20 NOK valid until 2025-06-27
Use promo code "24112" to get your promotional price.
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Description
The Human Interleukin-6 (Hu IL-6) ELISA quantitates Hu IL-6 in human serum, plasma, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-6. Principle of the method The Human IL-6 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.
Specifications
| P05231 | |
| <1 pg/mL | |
| HRP | |
| ELISA | |
| No significant cross-reaction with with human IL-1 A, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-10, IL-12, TNFa, IFNg, IFNa, GM-CSF, or mouse IL-6 | |
| Human | |
| 3569 | |
| <10% | |
| Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
| Absorbance at 450 nm minus absorbance (at 550 nm) | |
| 96 Tests | |
| Plasma 50 μL, Serum 50 μL, Supernatant 50 μL, Urine 50 μL | |
| IL-6 | |
| 3 hrs |
| 10.24 to 400 pg/mL | |
| Human IL-6 ELISA Kit | |
| Colorimetric ELISA (TMB substrate) with Plates | |
| Plasma, Serum, Supernatant, Urine | |
| 96 microplate wells | |
| Colorimetric Microplate Reader | |
| BSF-2,BSF2,CDF,HGF,HSF,IFN-beta-2,IFNB2,IL-6 | |
| <10 | |
| HRP | |
| Intra-assay CV <10%; inter-assay CV <10% | |
| RUO | |
| 2°C to 8°C | |
| 1 hrs 20 mins |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Human IL-6 ELISA Kit
Signal Word
- Warning
Hazard Category
- Skin sensitiser Category 1
Hazard Statement
- H317-May cause an allergic skin reaction.
Precautionary Statement
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P272-Contaminated work clothing should not be allowed out of the workplace.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
- P362+P364-Take off contaminated clothing and wash it before reuse.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : 2-methyl-3(2H)-isothiazolone
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