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  6. Cytiva HisTrap™ HP Prepacked Columns

Cytiva HisTrap™ HP Prepacked Columns

Product Code. 10431065 Shop All Cytiva Products
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Quantity:
1 mL
5 mL
5 x 1 mL
5 x 5 mL
Diameter (Metric) Inner:
7 mm
16 mm
Unit Size:
1mL
25mL
5mL
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

  • Prepacked with Ni Sepharose™ High Performance medium
  • Negligible nickel ion leakage
  • Pressure Max: 3 bar
  • Column material: polypropylene
  • 1 and 5mL columns
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
  • Manual operation with syringe, pump or chromatography system

Media
  • Matrix: 6% cross-linked agarose
  • Average particle size: 34μm
  • pH stability working range: 3 to 12

1mL columns

  • Bed Dimensions: 7 x 25mm
  • Flow velocity: 4mL/min.

5mL columns
  • Bed Dimensions: 16 x 25mm
  • Flow velocity: 5mL/min.

TRUSTED_SUSTAINABILITY

Specifications

Bed Volume 1 mL
Diameter (English) Inner 0.2 in.
Diameter (Metric) Inner 7 mm
Description HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Material Polypropylene
pH Range 3 to 12
Support Type 6% Highly Cross-linked Agarose
Content And Storage 4°C to 30°C
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
Quantity 5 x 1 mL
Type Pre-packed Column
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Safety and Handling

compliance-icons
Signal Word
  • Warning
 Hazard Category
  • Flammable liquid Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • EUH208-Contains . May produce an allergic reaction.
 Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P235-Keep cool.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : Ethanol, Nickel
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