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Thermo Scientific™ HisPur™ Cobalt Purification Kit, 3 mL

Product Code. 11894201 Shop All Thermo Scientific Products
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Quantity:
5 Column Kit
Unit Size:
3mL
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

Gently purify His-tagged recombinant fusion proteins using cobalt (Co2+) immobilized metal chelate affinity (IMAC) beads, spin columns and plates.

Thermo Scientific™ HisPur Cobalt Resin is an immobilized metal chelate affinity (IMAC) support for specific, fast and gentle His-tagged purification.

Additional Information and Online Ordering

HisPur Cobalt Resins, Columns and Kits use a tetradentate chelating resin charged with divalent cobalt (Co2+)to enable specific, fast and gentle purification of histidine-tagged fusion proteins from a variety of sample types. HisPur Cobalt Resin is available in several package sizes of resin slurry, three sizes of centrifuge-ready columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates for high throughput needs. With these options, high-quality His-tag protein purification is possible at nearly any scale. HisPur Cobalt Resin is superior to traditional nickel-based immobilized metal affinity chromatography (IMAC) systems. Compared to Ni-IDA and tetradentate Ni2+chelate resins, HisPur Resin binds histidine-tagged proteins with higher specificity (less off-target binding) and releases them with gentler conditions (lower concentrations of imidazole). Use HisPur Columns and Cartridges to obtain high-purity His-tagged proteins with no metal contamination.

Highlights:

  • High purity – obtain more than 10 milligrams of pure His-tagged protein per milliliter of resin without optimizing imidazole washing conditions

  • Low metal leaching – no metal contamination in eluted histidine-tagged protein sample

  • Versatility – purify proteins under native or denaturing conditions; compatible with cell lysis reagents and a variety of buffer additives

  • Flexibility – available as bulk resin or predispensed columns, chromatography columns and 96-well filter plates

  • Cost effective – resin can be reused several times or discarded after single use

  • Superior – performs better than other commercially available IMAC Resins

Available Formats:

  • Resin Slurries – crosslinked 6% beaded agarose; 10mL, 100mL, 500mL bottles
  • Spin Columns – 0.2mL (microcentrifuge), 1mL, 3mL columns
  • Purification Kits – complete kits in all three column sizes
  • Chromatography Cartridges – compatible with automated liquid chromatography systems and with syringe processing
  • 96-well Spin Plates – pre-dispensed 96-well filter plates, compatible with centrifugation and vacuum manifold systems for manual or automated purification. The plates enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput separations
  • Bulk and Custom – let us know your needs for bulk HisPur Cobalt Resin

Includes:

Kits contain HisPur Cobalt Columns, equilibration/wash buffer and elution buffer

TRUSTED_SUSTAINABILITY

Specifications

Capacity (Metric) 1 mL/Column
Description HisPur Cobalt Spin Purification Kit, 3mL
Content And Storage Sufficient For: Purifying ∼30 mg of His-tagged protein per column
• HisPur Cobalt Spin Columns, 3 mL, 5 columns
• Equilibration/Wash Buffer, 225 mL
• Elution Buffer, 75 mL

Store at 4°C.
Product Line HisPur™
Quantity 5 Column Kit
Target His-Tagged Protein
Type Protein Purification System
Stationary Phase Metal Chelate, Divalent Cobalt
Sufficient For 2 mg of His-Tagged Protein per column
Format Spin Column
Column Type Affinity, Agarose Resin
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Safety and Handling

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Product Identifier
  • HisPur Cobalt Purification Kit, 3 mL
 Signal Word
  • Danger
 Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Flammable liquid Category 3
  • Reproductive toxicity Category 1B
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • H319-Causes serious eye irritation.
  • H360-May damage fertility or the unborn child.
 Precautionary Statement
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P264-Wash hands thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P337+P313-If eye irritation persists: Get medical advice/attention.
  • P403+P235-Store in a well-ventilated place. Keep cool.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : Ethanol, 1-Imidazole, Sodium chloride

For Research Use Only. Not for use in diagnostic procedures.

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