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Cytiva HisPrep Column

Product Code. 10267934 Shop All Cytiva Products
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Volume (Metric):
20 mL
Unit Size:
Each
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This item is not returnable. View return policy

Description

Optimize purification of histidine-tagged recombinant proteins. Cytiva Life Sciences™ HisPrep Column is a ready to use column offering convenient scale-up purification of histidine-tagged proteins.

  • Optimized for convenient scale-up purification of histidine-tagged proteins
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
  • Negligible leakage of Ni2+
  • Bed Volume 20ml
  • High binding capacity
  • Convenient and time-saving prepacked HiPrep format with precharged Ni Sepharose 6 Fast Flow medium
  • Media:
  • BioProcess media
  • 90μm average particle size
  • Metrix 6% cross-linked agarose
  • pH stability working range 3-121
  • Metal ion capacity ∽15μmol Ni2+™/ml medium
  • Three years shelf life
  • Stable in commonly used aqueous buffers - 0.1 M HCl, 0.1 M NaOH, 8 M urea3
  • Column:
  • Inside Diameter is 16mm
  • Material of Column hardware and tube is Polypropylene and bed support is Polymethyl-Methacrylate
  • Bed support Filter

  • Bed Dimensions 16 x 100mm
  • Flow rate <10ml/min1
  • Flow Velocity <300cm/h
  • Pressure Max. 1.5 bar

TRUSTED_SUSTAINABILITY

Specifications

Diameter (English) Inner Column 0.6 in.
Flow Rate <10 mL/min.
Content And Storage 4°C to 30°C
Diameter (Metric) Inner Column 1.6 cm
Volume (Metric) 20 mL
Quantity Each

Safety and Handling

compliance-icons
Signal Word
  • Warning
 Hazard Category
  • Flammable liquid Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • EUH208-Contains . May produce an allergic reaction.
 Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P235-Keep cool.
  • P501-Dispose of contents/container to .
 Supplemental information
  • MIXTURE LIST-Contains : Nickel
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