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  7. Gibco™ RPMI 1640 Medium, GlutaMAX™ Supplement, HEPES

Gibco™ RPMI 1640 Medium, GlutaMAX™ Supplement, HEPES

Product Code. p-4919941
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Quantity:
5 L
10 L
Unit Size:
Each
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

Requires

Supplementation

Originally developed to culture human leukemic cells in suspension and as a monolayer

RPMI 1640 Medium (Roswell Park Memorial Institute 1640 medium) has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.

  • Modified as follows: with GlutaMAX™, phenol red, HEPES
  • Contains reducing agent glutathione and high concentrations of vitamins
  • Contains biotin, vitamin B12 and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
  • Vitamins inositol and choline are present in very high concentrations
  • GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (2.0g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH

Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Cell Culture

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Form Liquid
With Additives GlutaMAX, Phenol Red
Without Additives No Sodium Pyruvate
Quantity 10 L
Formulation Biotin, Calcium nitrate (Ca(NO3)2 4H2O), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Folic Acid, Glutathione (reduced), Glycine, HEPES, L-Alanyl-Glutamine, L-Arginine, L-Asparagine, L-Aspartic acid, L-Cystine, L-Glutamic Acid, L-Histidine, L-Hydroxyproline, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine, L-Valine, Magnesium Sulfate (MgSO4-7H2O), Niacinamide, Para-Aminobenzoic Acid, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4) anhydrous, Thiamine hydrochloride, Vitamin B12, i-Inositol
Content And Storage Storage: 2-8° C. Protect from light. Shelf life: 12 months from date of manufacture

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

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