missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Cell Culture Media, Supplements, and Reagents
  4. Cell Culture Media
  5. Classical and Basal Cell Culture Media
  6. Ham's F-12 Cell Culture Media
  7. Gibco™ Ham's F-12 Nutrient Mix 

Gibco™ Ham's F-12 Nutrient Mix
GREENER_CHOICE

Product Code. p-4919858
Click to view available options
Quantity:
500 mL
1000 mL
Unit Size:
1000mL
500mL
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

Requires

Supplementation

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells.

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Ham's F-12 is modified as follows:

With: L-glutamine, Phenol Red

The complete formulation is available.

Using F-12

Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Cell Line CHO, COS-7, and rat prostate epithelial cells
Cell Type Primary Rat Astrocytes
Classification Animal Origin-free
Concentration 1 X
Form Liquid
Product Type Ham's F-12 Nutrient Mixture
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives Glutamine, Phenol Red, Sodium Pyruvate
Without Additives No HEPES
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco™
Quantity 500 mL
Shipping Condition Room Temperature
Content And Storage Storage conditions: 2°C to 8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Show More Show Less
Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.