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Cytiva RNAspin 96 RNA Isolation Kit
For fast and efficient purification in a 96-well format
Brand: Cytiva 25-0500-75
Includes: Protocol and sufficient quantities of the following to carry out the number of preps indicated: DNase 1, RNase-free water, binding plates, 1.5mL tubes, wash plates, square well blocks, vacuum elution plates and buffers
Description
- 96 samples parallel-processed via either centrifugation or vacuum with high reproducibility in under 70 minutes
- Can be used to isolate total RNA from cultured cells, tissue, bacteria or yeast
- DNase I treatment removes genomic DNA resulting in high-quality total RNA product
- Isolated RNA is of sufficient quality (A260/A280 = 1.8-2.2) and quantity (up to 100μg) for multiple downstream experiments: quantitative reverse transcription polymerase chain reaction (QRT-PCR), primer extension and RNase protection assays
- Integrated wash plate eliminates risk of contamination
- Accessory tissue plate allows tissue samples to be processed
- Sample size: up to 30mg for animal tissue, up to 2 x 106 cells for cell culture (vaccum); 1x 107 cells for cell culture (centrifuge); Elution volume: 50μ-130μL
Specifications
| illustra RNAspin 96 Kit | |
| Used to reproducibly (well-to-well, plate-to-plate) isolate total RNA from cultured cells, tissue, bacteria or yeastCan also be used in a fully automated flow with vacuum on common laboratory workstationsSuitable for direct use in downstream application | |
| 4 x 96 Preps | |
| 1.8-2.2 | |
| 70 min/4 preps |
| 96-well Plate | |
| Protocol and sufficient quantities of the following to carry out the number of preps indicated, DNase 1, RNase-free Water, binding plates, 1.5mL tubes, wash plates, square well blocks, vacuum elution plates and Buffers | |
| illustra RNAspin 96 Kit | |
| 10 mg tissue-30 mg tissue |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Danger
Hazard Category
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
- Flammable liquid Category 3
- Respiratory sensitiser Category 1
- Skin sensitiser Category 1
Hazard Statement
- H226-Flammable liquid and vapour.
- H302-Harmful if swallowed.
- H312-Harmful in contact with skin.
- H317-May cause an allergic skin reaction.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H412-Harmful to aquatic life with long lasting effects.
Precautionary Statement
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P264-Wash thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : deoxyribose nuclease, thiocyanic acid, ethanol
- UNKNOWN ACUTE-27.5 per cent of the mixture consists of component(s) of unknown acute toxicity.
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