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Thermo Scientific™ EZ-Link™ Alkoxyamine-PEG4-Biotin
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Thermo Scientific™ EZ-Link Alkoxyamine-PEG4-Biotin is a biotinylation reagent containing a four-unit polyethylene glycol (PEG) spacer for biotinylating macromolecules at carbohydrate groups that have been oxidized to form aldehydes.
Highlights:
Biotin – label macromolecules for detection or affinity purification with avidin and streptavidin
Alkoxyamine-activated – reacts with aldehyde groups (e.g., oxidized sugars in glycoproteins) to attach biotin by stable oxime bonds
Polyethylene glycol – PEG groups are flexible, non-immunogenic, hydrophilic, and improve the solubility of labeled antibodies
Spacer arm – the reach between the point of attachment and the biotin affinity tag is nearly 27 angstroms, sufficient to minimizing steric hindrance for binding
Specifications:
- Chemical formula: C18H34N4O6S
- Molecular weight: 434.55
- Spacer arm: 27.0 angstroms
- Net mass added to target: 416.53
- Form: white solid
- Solubility: dissolve in DMSO to make 250mM stock

Specifications
Specifications
Product Type | Alkoxyamine-PEG4-Biotin |
Quantity | 50 mg |
Content And Storage | Store at -20°C. Shipped at ambient temperature. |
Product Line | EZ-Link™ |
Cell Permeability | Cell-Impermeant |
Chemical Reactivity | Ketone, Aldehyde |
Reactive Moiety | Alkoxyamine |
Solubility | DMF (Dimethylformamide), DMSO (Dimethylsulfoxide), Water |
Label or Dye | Biotin |
Spacer | Long, Pegylated |
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For Research Use Only. Not for use in diagnostic procedures.
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