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Thermo Scientific™ DyLight™ 755 Maleimide
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Quantity:
1 mg
Unit Size:
1mg
Description
Thermo Scientific™ Sulfhydryl-Reactive DyLight Dyes are maleimide-activated DyLight Fluors for fluorescent labeling of antibodies and other proteins to be used as probes for cellular imaging and other fluorescence detection methods.
The Thermo Scientific™ DyLight™ Dyes have absorption spectra ranging from 350 to 770nm. These reagents fluoresce over a broad pH range, are more intense than Alexa Fluor™ or Cy™ Dyes in many applications and match the output wavelengths of common fluorescence instrumentation. Additionally, the water solubility of the DyLight Reagents allows a high fluor-to-protein ratio without precipitation during conjugation.
The sulfhydryl-reactive dyes contain maleimide groups that react predominantly with free –SH groups at pH 6.5-7.5, forming a stable thioether bond. At pH 7, the maleimide group is ∼1000 times more reactive toward a free sulfhydryl than to an amine.1At pH values > 7.5, reactivity toward primary amines increases and hydrolysis of the maleimide group can occur.
Highlights:
Applications:
The sulfhydryl-reactive dyes contain maleimide groups that react predominantly with free –SH groups at pH 6.5-7.5, forming a stable thioether bond. At pH 7, the maleimide group is ∼1000 times more reactive toward a free sulfhydryl than to an amine.1At pH values > 7.5, reactivity toward primary amines increases and hydrolysis of the maleimide group can occur.
Highlights:
- Specific — maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH)
- Efficient labeling methods — well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling
- Optimized antibody labeling procedure —complete protocol for IgG reduction and labeling and calculating the labeling efficiency
Applications:
- Antibody labeling for immunofluorescence applications, including immunocytochemistry (ICC), immunohistochemistry (IHC), Western blotting and ELISA assay
- Target macromolecule labeling for in vitro and in vivo fluorescent detection strategies
Specifications
Specifications
| Description | DyLight 755 Maleimide |
| Quantity | 1 mg |
| Product Type | Dye |
| Solubility | DMF (Dimethylformamide) |
| Content And Storage | Store desiccated at -20°C. |
| Chemical Reactivity | Thiol |
| Reactive Moiety | Maleimide |
| Label or Dye | DyLight™ 755 |
| Label Type | DyLight Fluors |
| Product Line | DyLight™ |
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For Research Use Only. Not for use in diagnostic procedures.
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