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MTCO1 Monoclonal Antibody (1D6E1A8), Invitrogen™

Mouse Monoclonal Antibody

Brand: Invitrogen 459600

673.00 CHF valid until 2025-05-31
Use promo code "25977" to get your promotional price.

Product Code. 10444035

740.00CHF / Each

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Description

For best results with this antibody in Western blot, do not boil samples before loading onto the gel. Boiling of the sample will cause a loss of signal. Hydrophobic intrinsic membrane proteins such as the core mtDNA-encoded proteins of the mitochondrial OXPHOS complexes tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio. In Western blot applications, this antibody detects a band at approximately 40 kDa (predicted MW: 57 kDa). In mouse liver lysate a specific band below 37 kDa was detected. Recommended positive controls: ICC/IF - Pig retinal pigment epithelial cells, rat cerebellum primary cells IHC (P) - Human skeletal muscle and colon tissue, rat pancreas tissue, pig smooth muscle tissue WB - Mouse, human, bovine and rat heart mitochondria lysate Flow - HEK-293 cells Recommended long term storage at -20°C.

MTCO1 Monoclonal antibody specifically detects MTCO1 in Human, Mouse, Rat samples. It is validated for Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot.

Specifications

Antigen	MTCO1
Classification	Monoclonal
Concentration	1 mg/mL
Formulation	59% PBS with 0.05% BSA, 40% glycerol and 0.01% sodium azide
Gene Accession No.	P05503, P00395, Q36347, O79876, P00397, Q9MIY8
Gene Symbols	COX1, MT-CO1
Immunogen	Human Mitochondrial Complex IV subunit I
Quantity	100 μg
Primary or Secondary	Primary
Target Species	Human, Mouse, Rat
Product Type	Antibody
Isotype	IgG2a κ

Applications	Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Clone	1D6E1A8
Conjugate	Unconjugated
Gene	MT-CO1
Gene Alias	Co1; coi; cox1; coxi; Cytochrome c oxidase polypeptide I; Cytochrome c oxidase subunit 1; cytochrome c oxidase subunit I; mitochondrially encoded cytochrome c oxidase I; mtco1; mt-co1
Host Species	Mouse
Purification Method	Protein A
Regulatory Status	RUO
Gene ID (Entrez)	140539, 17708, 26195, 4512, 1485858, 808503
Content And Storage	4° C, do not freeze
Form	Liquid

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MTCO1 Monoclonal Antibody (1D6E1A8), Invitrogen™

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