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Invitrogen™ RiboPure™ RNA Purification Kit, yeast
For the isolation of extremely high-quality RNA from yeast without the use of enzymatic pretreatments that may alter gene expression profiles.
Brand: Invitrogen™ AM1926
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Description
For the isolation of extremely high-quality RNA from yeast without the use of enzymatic pretreatments that may alter gene expression profiles. Each Invitrogen kit includes sufficient reagents for 50 purifications each from 107 to 108 yeast cells.
It has traditionally been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Many protocols use enzymatic pretreatments that may affect gene expression patterns. The RiboPure-Yeast RNA Isolation Kit uses no enzymatic pretreatments and thus does not alter expression profiles. It combines a simple and efficient glass bead/one step phenol–mediated disruption using Invitrogen's Vortex Bead Beater Adapter followed by a glass fiber filter– based RNA purification step. After DNase treatment with the included DNA-free™ Reagents, the resulting RNA is exceptionally pure containing little to no genomic DNA (less than 0.05%). The kit has been used extensively at Invitrogen to isolate high-quality RNA from Saccharomyces cerevisiae, Schizosaccharomyces pombe, Pichia pastoris, and Candida albicans. The kit provides the highest yield of any method tested from each of these species.
- Highest yields of total RNA available
- No enzymatic pretreatment required
- Includes DNA-free™ Reagents
Not high-throughput compatible (Manual)
Applications:
DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Yeast
Specifications
| • 50 1.5-mL Screw Cap Tubes; store at room temperature • 50 Filter Cartridges; room temperature • 100 Collection Tubes (2 mL); room temperature • 24 mL Lysis Buffer; 4°C • 2.4 mL 10% SDS; 4°C • 24 mL Phenol:Chloroform:IAA; 4°C • 95 mL Binding Buffer; 4°C • 35 mL Wash Solution 1; 4°C • 50 mL Wash Solution 2/3 (concentrated); 4°C • 5 mL Elution Solution; 4°C • 80 g Zirconia Beads; -20°C • 550 μL 10X DNase 1 Buffer; -20°C • 200 μL DNase 1; -20°C • 550 μL DNase Inactivation Reagent; -20°C |
|
| 1.5 hr. (Including DNase treatment) | |
| Yeast | |
| Not High-throughput Compatible (Manual) | |
| 50 Preps | |
| Up to 108 cells |
| Spin Column (Glass Fiber Filter) | |
| 50–100 μL | |
| Total RNA | |
| RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling | |
| Box 1: Dry Ice Box 2: Room Temperature |
|
| Up to 300 μg |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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