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Invitrogen™ mMESSAGE mMACHINE™ T7 Transcription Kit
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Quantity:
25 Reactions
Unit Size:
25 reactions
Description
mMESSAGE mMACHINE™ kits are designed for the in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5′ end.
- RNA Yield: 20-30μg
- Include cap analog [m7G(5′)ppp(5′)G] in ultra high-yield transcription reaction
- Simplified reaction format in which all four ribonucleotides and cap analog are mixed in single solution
- Cap analog: GTP ratio of solution is 4:1, which is optimal for maximizing both RNA yield and the proportion of capped transcripts
- For routine synthesis of capped RNAs for oocyte microinjection, in vitro translation, transfection, and other applications
- High yields are achieved by optimizing reaction conditions for RNA synthesis in presence of high nucleotide concentrations
- RNA synthesized is protected from degradation by contaminating ribonucleases that present with RNase inhibitor - component of the Enzyme Mix
Specifications
Specifications
| Content And Storage | T7 Enzyme Mix, 10X Reaction Buffer 2XNTP⁄CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature. |
| Final Product Type | Synthetic mRNA, mRNA |
| Format | Kit |
| Sample Type | mRNA, Synthetic mRNA |
| For Use With (Application) | In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products |
| No. of Reactions | 25 Reactions |
| Product Line | Ambion™, mMESSAGE mMACHINE™ |
| Product Type | T7 In Vitro Transcription kit |
| Quantity | 25 Reactions |
| Promoter | T7 |
| Show More |
Safety and Handling
Product Identifier
- mMESSAGE mMACHINE T7 Transcription Kit
- Danger
- Acute toxicity Category 4
- Carcinogenicity Category 2
- Serious eye damage/eye irritation Category 2
- Reproductive toxicity Category 1B
- Skin corrosion/irritation Category 2
- Specific target organ toxicity after repeated exposure Category 2
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H351-Suspected of causing cancer.
- H360-May damage fertility or the unborn child.
- H373-May cause damage to organs.
- P201-Obtain special instructions before use.
- P202-Do not handle until all safety precautions have been read and understood.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P314-Get medical advice/attention if you feel unwell.
- P330-Rinse mouth.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : lithium chloride, formamide
For Research Use Only. Not for use in diagnostic procedures.
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