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2-Mercaptoethanol, Electrophoresis Grade, 98+%
Description
β-Mercaptoethanol can act as an enzyme reactivator in systems necessitating reduction for activation, and has been commonly used to reduce disulfide bonds in order to separate protein subunits for use in electrophoresis. This product can also be used to form o-Phthalaldehyde-beta-mercaptoethanol (OPAME), a fluorogenic reagent shown to detect amino acids based on its reaction with primary amines. More recently OPAME has been used to distinguish between ssDNA/dsDNA and even single base mismatches in the hybridization of DNA. It is widely used for retarding oxidation of biological compounds in solution.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
β-Mercaptoethanol can act as an enzyme reactivator in systems necessitating reduction for activation, and has been commonly used to reduce disulfide bonds in order to separate protein subunits for use in electrophoresis. This product can also be used to form o-Phthalaldehyde-beta-mercaptoethanol (OPAME), a fluorogenic reagent shown to detect amino acids based on its reaction with primary amines. More recently OPAME has been used to distinguish between ssDNA/dsDNA and even single base mismatches in the hybridization of DNA. It is widely used for retarding oxidation of biological compounds in solution.
Solubility
Fully miscible in water. Soluble in alcohol, ether, benzene, and other organic solvents.
Notes
Handle under dry protective gas. Store in cool condition. Incompatible with air, moisture, oxidizing agents.
Specifications
Specifications
| Melting Point | -100°C |
| Color | Colorless |
| Density | 1.114 g/mL |
| Boiling Point | 156°C to 158°C |
| Flash Point | 73°C (163°F) |
| Odor | Unpleasant |
| Linear Formula | HSCH2CH2OH |
| Quantity | 1000 g |
| UN Number | UN2966 |
| Beilstein | 773648 |
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Safety and Handling
- Danger
- Acute toxicity Category 2
- Acute toxicity Category 3
- LONG-TERM AQUATIC HAZARD Chronic 2
- ACUTE AQUATIC Acute 1
- Serious eye damage/eye irritation Category 1
- Reproductive toxicity Category 2
- Skin corrosion/irritation Category 2
- Skin sensitiser Category 1
- Specific target organ toxicity after repeated exposure Category 2
- H301-Toxic if swallowed.
- H310-Fatal in contact with skin.
- H315-Causes skin irritation.
- H317-May cause an allergic skin reaction.
- H318-Causes serious eye damage.
- H331-Toxic if inhaled.
- H361f-Suspected of damaging fertility.
- H373-May cause damage to organs.
- H400-Very toxic to aquatic life.
- H411-Toxic to aquatic life with long lasting effects.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
- P302+P352-IF ON SKIN: Wash with plenty of water/.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P310-Immediately call a POISON CENTER/doctor/.
RUO – Research Use Only
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